The development and implementation of the GEMM-ESC approach in our transgenic core facility minimizes the breeding needs. Experimental cohorts are generated on-demand and ready-to-use, thereby reducing the number of mice needed per experiment.
In 2017, the MCCA transgenic facility acquired the
This technology is based on the use of goGermline blastocysts for injection of embryonic stem cells (ESC).
This new technology eliminates the competition with the host blastocysts and results in 100% ESC derived offspring.
In 2017 it was awarded
the ISTT 3Rs prize.
Embryo transfer of injected embryos into foster mothers is a crucial step in the generation of new mouse models.
Typically this is performed by surgical procedure. Since 2011, we have replaced this invasive procedure with the routine use of the Non-Surgical Embryo Transfer (NSET) with great success, minimizing distress for the foster mothers. For this effort we were awarded the national price for “Alternatives for Animal Testing 2013”.
The procedure is described in our Transgenic Research publication of 2014.
When planning animal experiments alternative methods that yield the same quality of data are carefully considered.